Type

Journal Article

Authors

R Paul Ross
Colin Hill
Paul D. Cotter
Des Field
Pat G. Casey
Alicia Campion

Subjects

Pharmacology

Topics
nisin mice drug effects whole body imaging pharmacology mice inbred balb c treatment outcome animals anti bacterial agents female listeria monocytogenes drug therapy administration dosage listeriosis

In vivo activity of nisin A and nisin V against Listeria monocytogenes in mice. (2012)

Abstract Lantibiotics are post-translationally modified antimicrobial peptides, of which nisin A is the most extensively studied example. Bioengineering of nisin A has resulted in the generation of derivatives with increased in vitro potency against Gram-positive bacteria. Of these, nisin V (containing a Met21Val change) is noteworthy by virtue of exhibiting enhanced antimicrobial efficacy against a wide range of clinical and food-borne pathogens, including Listeria monocytogenes. However, this increased potency has not been tested in vivo. Here we address this issue by assessing the ability of nisin A and nisin V to control a bioluminescent strain of Listeria monocytogenes EGDe in a murine infection model.More specifically, Balb/c mice were infected via the intraperitoneal route at a dose of 1 × 10(5) cfu/animal and subsequently treated intraperitoneally with either nisin V, nisin A or a PBS control. Bioimaging of the mice was carried out on day 3 of the trial. Animals were then sacrificed and levels of infection were quantified in the liver and spleen. This analysis revealed that nisin V was more effective than Nisin A with respect to controlling infection and therefore merits further investigation with a view to potential chemotherapeutic applications.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

R Paul Ross, Colin Hill, Paul D. Cotter, Des Field, Pat G. Casey, Alicia Campion

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Paul D. Cotter
Teagasc
Total Publications: 253
 
4
Des Field
Teagasc
Total Publications: 30
 
5
Pat G. Casey
University College Cork
Total Publications: 40
 
6
Alicia Campion
University College Cork
Total Publications: 5