Type

Journal Article

Authors

Douwe van Sinderen
Finn K Vogensen
Bashir Aideh
Knut J Heller
Horst Neve
Robert A Britton
Jan-Peter van Pijkeren
Marie-Pierre Chapot-Chartier
Pascal Courtin
Jennifer Mahony
and 1 others

Subjects

Agriculture & Food Science

Topics
stationary phase fibre protein processing tail cell wall infection phages specialization

The lactococcal phages Tuc2009 and TP901-1 incorporate two alternate forms of their tail fibre into their virions for infection specialization. (2013)

Abstract Lactococcal phages Tuc2009 and TP901-1 possess a conserved tail fibre, called a Tail-Associated Lysin (referred to as Tal2009 for Tuc2009, and Tal901-1 for TP901-1), suspended from their tail tips that projects a Peptidoglycan-Hydrolase (PGH) domain towards a potential host bacterium. Tal2009 and Tal901-1 can undergo proteolytic processing mid-protein at the glycine-rich sequence GG(S/N)SGGG, removing their C-terminal structural lysin. In this study, we show that the PGH of these Tal proteins is an M23 peptidase which exhibits D-Ala-D-Asp endopeptidase activity, and that this activity is required for efficient infection of stationary phase cells. Interestingly, the observed proteolytic processing of Tal2009 and Tal901-1 facilitates increased host adsorption efficiencies of the resulting phages. This represents, to the best of our knowledge, the first example of tail fibre proteolytic processing that results in a heterogeneous population of two phage types. Phages which possess a full-length tail fibre, or a truncated derivative, are better adapted to efficiently infect cells with extensively cross-linked cell wall, or infect with increased host-adsorption efficiencies, respectively.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Finn K Vogensen, Bashir Aideh, Knut J Heller, Horst Neve, Robert A Britton, Jan-Peter van Pijkeren, Marie-Pierre Chapot-Chartier, Pascal Courtin, Jennifer Mahony and 1 others

Experts in our system

1
Knut J Heller
University College Cork
 
2
Horst Neve
University College Cork
Total Publications: 41
 
3
Marie-Pierre Chapot-Chartier
University College Cork
 
4
Pascal Courtin
University College Cork
 
5
Jennifer Mahony
University College Cork
Total Publications: 87