Type

Journal Article

Authors

R Paul Ross
Paul D. Cotter
Colin Hill
Lucy H Deegan
Lorraine A Draper

Subjects

Biochemistry

Topics
drug effects bacteriocins mutagenesis site directed pharmacology bioengineering structure activity relationship bacteria bacterial proteins anti bacterial agents atp binding cassette transporters genetics membrane proteins metabolism

Insights into Lantibiotic Immunity Provided by Bioengineering of LtnI. (2012)

Abstract The lantibiotic lacticin 3147 has been the focus of much research due to its broad spectrum of activity against many microbial targets, including drug-resistant pathogens. In order to protect itself, a lacticin 3147 producer must possess a cognate immunity mechanism. Lacticin 3147 immunity is provided by an ABC transporter, LtnFE, and a dedicated immunity protein, LtnI, both of which are capable of independently providing a degree of protection. In the study described here, we carried out an in-depth investigation of LtnI structure-function relationships through the creation of a series of fusion proteins and LtnI determinants that have been the subject of random and site-directed mutagenesis. We establish that LtnI is a transmembrane protein that contains a number of individual residues and regions, such as those between amino acids 20 and 27 and amino acids 76 and 83, which are essential for LtnI function. Finally, as a consequence of the screening of a bank of 28,000 strains producing different LtnI derivatives, we identified one variant (LtnI I81V) that provides enhanced protection. To our knowledge, this is the first report of a lantibiotic immunity protein with enhanced functionality.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

R Paul Ross, Paul D. Cotter, Colin Hill, Lucy H Deegan, Lorraine A Draper

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Paul D. Cotter
Teagasc
Total Publications: 253
 
3
Colin Hill
University College Cork
Total Publications: 351
 
4
Lorraine A Draper
University College Cork
Total Publications: 24