Type

Journal Article

Authors

Cormac G M Gahan
Colin Hill
Pat G. Casey
Matthias Eberl
Martin S. Davey
Maire Begley
Sinéad Heuston

Subjects

Microbiology

Topics
animals genes bacterial biosynthetic pathways genetics mevalonic acid microbiology metabolism listeria monocytogenes listeriosis liver enzymology gene deletion genetic complementation test growth development enzymes pathology mice disease models animal spleen genes essential terpenes bacterial proteins

HmgR, a key enzyme in the mevalonate pathway for isoprenoid biosynthesis, is essential for growth of Listeria monocytogenes EGDe. (2012)

Abstract Isoprenoids may be synthesized via one of two pathways, the classical mevalonate pathway or the alternative 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. While the majority of bacteria utilize a single pathway for isoprenoid biosynthesis, Listeria monocytogenes is unusual in possessing the complete set of genes for both pathways. Here, we utilized new molecular tools to create precise gene deletions in selected genes encoding enzymes of both pathways, gcpE, lytB (encoding proteins in the MEP pathway) and hmgR (encoding a protein in the mevalonate pathway). We demonstrate that the hmgR gene can only be deleted when the growth medium is supplemented with exogenous mevalonate. Furthermore, full growth of the mutant in the absence of mevalonate was only possible when the intact hmgR gene was supplied in trans using an IPTG-inducible expression system. Murine competitive index assays performed via the oral and intraperitoneal routes of infection revealed that the mevalonate hmgR mutant could not be recovered from livers and spleens 3 days post-infection. We propose that HmgR in L. monocytogenes EGDe is involved in essential metabolic functions and that an intact MEP pathway is not capable of sustaining growth.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Cormac G M Gahan, Colin Hill, Pat G. Casey, Matthias Eberl, Martin S. Davey, Maire Begley, Sinéad Heuston

Experts in our system

1
Cormac G M Gahan
University College Cork
Total Publications: 109
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Pat G. Casey
University College Cork
Total Publications: 40
 
4
Matthias Eberl
University College Cork
Total Publications: 4
 
5
Maire Begley
University College Cork
Total Publications: 27