Type

Journal Article

Authors

Cormac G M Gahan
Phillip E. Klebba
Colin Hill
Richard E. Cowart
Susan A. Joyce
Roy D. Sleator
Alain Charbit
Trevor Darby
Pat G. Casey
Hualiang Pi
and 3 others

Subjects

Microbiology

Topics
genetics listeria monocytogenes gene expression regulation bacterial mutagenesis insertional genes bacterial molecular sequence data pathogenicity moths virulence mutation drug resistance bacterial computational biology biological assay plasmids base sequence genetic loci animals microbiology repressor proteins heme enzymology adenosine triphosphatases mice homeostasis bacterial proteins metabolism real time polymerase chain reaction listeriosis iron toxicity drug effects ferric uptake regulating proteins bacterial

A putative P-type ATPase required for virulence and resistance to haem toxicity in Listeria monocytogenes. (2011)

Abstract Regulation of iron homeostasis in many pathogens is principally mediated by the ferric uptake regulator, Fur. Since acquisition of iron from the host is essential for the intracellular pathogen Listeria monocytogenes, we predicted the existence of Fur-regulated systems that support infection. We examined the contribution of nine Fur-regulated loci to the pathogenicity of L. monocytogenes in a murine model of infection. While mutating the majority of the genes failed to affect virulence, three mutants exhibited a significantly compromised virulence potential. Most striking was the role of the membrane protein we designate FrvA (Fur regulated virulence factor A; encoded by frvA [lmo0641]), which is absolutely required for the systemic phase of infection in mice and also for virulence in an alternative infection model, the Wax Moth Galleria mellonella. Further analysis of the ΔfrvA mutant revealed poor growth in iron deficient media and inhibition of growth by micromolar concentrations of haem or haemoglobin, a phenotype which may contribute to the attenuated growth of this mutant during infection. Uptake studies indicated that the ΔfrvA mutant is unaffected in the uptake of ferric citrate but demonstrates a significant increase in uptake of haem and haemin. The data suggest a potential role for FrvA as a haem exporter that functions, at least in part, to protect the cell against the potential toxicity of free haem.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Cormac G M Gahan, Phillip E. Klebba, Colin Hill, Richard E. Cowart, Susan A. Joyce, Roy D. Sleator, Alain Charbit, Trevor Darby, Pat G. Casey, Hualiang Pi and 3 others

Experts in our system

1
Cormac G M Gahan
University College Cork
Total Publications: 109
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Susan A. Joyce
University College Cork
Total Publications: 42
 
4
Roy D. Sleator
University College Cork
Total Publications: 44
 
5
Trevor Darby
University College Cork
Total Publications: 4
 
6
Pat G. Casey
University College Cork
Total Publications: 40