Type

Journal Article

Authors

Paul D. Cotter
Gerald F Fitzgerald
Colin Hill
R Paul Ross
Catherine Stanton
Paula M. O'Connor
Sarah Norberg

Subjects

Microbiology

Topics
caseicin b caseicin a microbial sensitivity tests hydrophobic and hydrophilic interactions gram negative bacteria chemistry amino acid sequence peptide fragments antimicrobial cationic peptides amino acid substitution drug effects caseins molecular sequence data chemical synthesis gram positive bacteria staphylococcus aureus

Extensive manipulation of caseicins A and B highlights the tolerance of these antimicrobial peptides to change. (2012)

Abstract Caseicins A and B are low-molecular-weight antimicrobial peptides which are released by proteolytic digestion of sodium caseinate. Caseicin A (IKHQGLPQE) is a nine-amino-acid cationic peptide, and caseicin B (VLNENLLR) is a neutral eight-amino-acid peptide; both have previously been shown to exhibit antibacterial activity against a number of pathogens, including Cronobacter sakazakii. Previously, four variants of each caseicin which differed subtly from their natural counterparts were generated by peptide synthesis. Antimicrobial activity assays revealed that the importance of a number of the residues within the peptides was dependent on the strain being targeted. In this study, this engineering-based approach was expanded through the creation of a larger collection of 26 peptides which are altered in a variety of ways. The investigation highlights the generally greater tolerance of caseicin B to change, the fact that changes have a more detrimental impact on anti-Gram-negative activity, and the surprising number of variants which exhibit enhanced activity against Staphylococcus aureus.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Paul D. Cotter, Gerald F Fitzgerald, Colin Hill, R Paul Ross, Catherine Stanton, Paula M. O'Connor, Sarah Norberg

Experts in our system

1
Paul D. Cotter
Teagasc
Total Publications: 253
 
2
Gerald F Fitzgerald
Teagasc
Total Publications: 207
 
3
Colin Hill
University College Cork
Total Publications: 351
 
4
R Paul Ross
Teagasc
Total Publications: 441
 
5
Catherine Stanton
Teagasc
Total Publications: 261
 
6
Paula M. O'Connor
Teagasc
Total Publications: 85