Type

Journal Article

Authors

Cormac G M Gahan
Colin Hill
Maire Begley
Pat G. Casey
Georgina C Dowd

Subjects

Biochemistry

Topics
humans metabolism gene expression regulation bacterial bacterial proteins repressor proteins female zinc animals pathogenicity genetics mice inbred balb c microbiology listeria monocytogenes listeriosis mice growth development

Investigation of the role of ZurR in the physiology and pathogenesis of Listeria monocytogenes. (2011)

Abstract Listeria monocytogenes is a Gram positive pathogen that is ubiquitous in the environment. It is a facultative anaerobic rod that causes listeriosis, a disease with potentially lethal consequences for susceptible individuals. During infection, the pathogen is capable of sequestering metal ions to act as vital biocatalysts in cellular processes. The zinc uptake regulator (ZurR) is predicted to coordinate uptake of zinc from the external environment. An in-frame deletion of the zurR gene resulted in a mutant exhibiting a small colony phenotype and a smaller cell size. The zurR mutant was unaffected under conditions of zinc limitation but demonstrated increased sensitivity to toxic levels of zinc. The mutant also demonstrated a significant (1-log) reduction in virulence potential in the murine model of infection. Using a bioinformatic approach, we identified a number of potentially Zur-regulated genes in the genome of L. monocytogenes. Quantitative RT-PCR demonstrated significant de-repression of zurA, lmo0153, and lmo1671 in the zurR mutant background indicating that these putative transporters are ZurR regulated.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Cormac G M Gahan, Colin Hill, Maire Begley, Pat G. Casey, Georgina C Dowd

Experts in our system

1
Cormac G M Gahan
University College Cork
Total Publications: 109
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Maire Begley
University College Cork
Total Publications: 27
 
4
Pat G. Casey
University College Cork
Total Publications: 40
 
5
Georgina C Dowd
University College Cork
Total Publications: 3