Journal Article


C Hill
G F Fitzgerald
A L Kelly
R D Sleator
K M Considine



pressure food handling metabolism sequence deletion pharmacology culture media drug effects microbial viability genetics listeria monocytogenes food microbiology biosynthesis betaine carnitine proline food safety

A role for proline synthesis and transport in Listeria monocytogenes barotolerance. (2011)

Abstract To assess the contribution of proline biosynthesis to listerial barotolerance. Using a Listeria monocytogenes proBA deletion mutant, incapable of synthesizing proline, together with a proline-overproducing strain, the contribution of proline synthesis to listerial barotolerance was determined. The ΔproBA strain does not survive as well as the wild type when subjected to treatment of 500 MPa in rich media and 400 MPa in minimal media (c. 1 log lower survival in both conditions). Betaine and carnitine decrease the ability of the wild type to survive at low pressures (300 MPa), but confer normal or slightly increased levels of protection at higher pressures (350 and 400 MPa). A functional proline synthesis system is required for optimal survival of Listeria following treatment at high-pressure (HP) levels (500 MPa in brain heart infusion and 400 MPa in defined medium), particularly where other compatible solutes are absent or limiting.   Given the potential of HP processing as an effective food processing/safety strategy, understanding how pathogens such as Listeria have evolved to cope with such stresses is an important food safety consideration. In this context, the work presented here may help to develop safer and more effective processing regimens.
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Full list of authors on original publication

C Hill, G F Fitzgerald, A L Kelly, R D Sleator, K M Considine

Experts in our system

Colin Hill
University College Cork
Total Publications: 351
Gerald F Fitzgerald
Total Publications: 207
Alan L. Kelly
University College Cork
Total Publications: 62
Roy D. Sleator
University College Cork
Total Publications: 44
Kiera Considine
University College Cork