Type

Journal Article

Authors

R Paul Ross
Colin Hill
Paul D. Cotter
Karen Daly
Mary C. Rea
Paula M. O'Connor
Lisa Quigley
Des Field

Subjects

Microbiology

Topics
microbial viability genetics gram positive bacteria pharmacology anti bacterial agents amino acid substitution microbial sensitivity tests drug effects growth development nisin bacteriocins

Studies with bioengineered Nisin peptides highlight the broad-spectrum potency of Nisin V. (2010)

Abstract Nisin A is the most thoroughly investigated member of the lantibiotic family of antimicrobial peptides. In addition to a long history of safe use as a food antimicrobial, its activity against multi-drug resistant pathogens has resulted in a renewed interest in applying nisin as a chemotherapeutic to treat bacterial infections. The wealth of Nisin-related information that has been generated has also led to the development of the biotechnological capacity to engineer novel Nisin variants with a view to improving the function and physicochemical properties of this already potent peptide. However, the identification of bioengineered Nisin derivatives with enhanced antimicrobial activity against Gram-positive targets is a recent event. In this study, we created stable producers of the most promising derivatives of Nisin A generated to date [M21V (hereafter Nisin V) and K22T (hereafter Nisin T)] and assessed their potency against a range of drug-resistant clinical, veterinary and food pathogens. Nisin T exhibited increased activity against all veterinary isolates, including streptococci and staphylococci, and against a number of multi-drug resistant clinical isolates including MRSA, but not vancomycin-resistant enterococci. In contrast, Nisin V displayed increased potency against all targets tested including hVISA strains and the hyper-virulent Clostridium difficile ribotype 027 and against important food pathogens such as Listeria monocytogenes and Bacillus cereus. Significantly, this enhanced activity was validated in a model food system against L. monocytogenes. We conclude that Nisin V possesses significant potential as a novel preservative or chemotherapeutic compound.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

R Paul Ross, Colin Hill, Paul D. Cotter, Karen Daly, Mary C. Rea, Paula M. O'Connor, Lisa Quigley, Des Field

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Paul D. Cotter
Teagasc
Total Publications: 253
 
4
Karen M. Daly
Teagasc
Total Publications: 10
 
5
Mary C. Rea
Teagasc
Total Publications: 68
 
6
Paula M. O'Connor
Teagasc
Total Publications: 85
 
7
Lisa Quigley
Teagasc
 
8
Des Field
Teagasc
Total Publications: 30