Type

Journal Article

Authors

R Paul Ross
Paul D. Cotter
Colin Hill
Andreas Peschel
Floor Hugenholtz
Lorraine A Draper
Elaine M. Lawton
Srinivas Suda
Lucy H Deegan

Subjects

Microbiology

Topics
bacteriocins staphylococcus aureus pharmacology drug effects protein conformation lactococcus lactis mutagenesis site directed structure activity relationship lacticin 481 models molecular genetics anti bacterial agents microbial sensitivity tests

Manipulation of charged residues within the two-peptide lantibiotic lacticin 3147. (2009)

Abstract Lantibiotics are antimicrobial peptides which contain a high percentage of post-translationally modified residues. While most attention has been paid to the role of these critical structural features, evidence continues to emerge that charged amino acids also play a key role in these peptides. Here 16 'charge' mutants of the two-peptide lantibiotic lacticin 3147 [composed of Ltnα (2+, 2-) and Ltnβ (2+)] were constructed which, when supplemented with previously generated peptides, results in a total bank of 23 derivatives altered in one or more charged residues. When examined individually, in combination with a wild-type partner or, in some instances, in combination with one another, these mutants reveal the importance of charge at specific locations within Ltnα and Ltnβ, confirm the critical role of the negatively charged glutamate residue in Ltnα and facilitate an investigation of the contribution of positively charged residues to the cationic Ltnβ. From these investigations it is also apparent that the relative importance of the overall charge of lacticin 3147 varies depending on the target bacteria and is most evident when strains with more negatively charged cell envelopes are targeted. These studies also result in, for the first time, the creation of a derivative of a lacticin 3147 peptide (LtnβR27A) which displays enhanced specific activity.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

R Paul Ross, Paul D. Cotter, Colin Hill, Andreas Peschel, Floor Hugenholtz, Lorraine A Draper, Elaine M. Lawton, Srinivas Suda, Lucy H Deegan

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Paul D. Cotter
Teagasc
Total Publications: 253
 
3
Colin Hill
University College Cork
Total Publications: 351
 
4
Lorraine A Draper
University College Cork
Total Publications: 24
 
5
Elaine M. Lawton
University College Cork
Total Publications: 12