Type

Journal Article

Authors

R Paul Ross
Colin Hill
Paul D. Cotter
Maire Begley

Subjects

Microbiology

Topics
nisin deficiency pharmacology drug effects gene deletion models biological enzymology microbial viability glutamate decarboxylase metabolism anti bacterial agents listeria monocytogenes drug resistance bacterial food preservatives growth development

Glutamate decarboxylase-mediated nisin resistance in Listeria monocytogenes. (2010)

Abstract Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (ΔgadD1, ΔgadDT1, ΔgadD2, ΔgadT2, and ΔgadD3 mutants) revealed that the ΔgadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain variable and is found only in approximately 50% of L. monocytogenes strains. Growth and survival experiments revealed that possession of gadD1 correlates with a higher degree of tolerance to nisin. Significantly, a similar finding using a gadB mutant of L. lactis IL1403 implies that this may be a general phenomenon in Gram-positive bacteria. Our findings thus suggest that the specific inhibition of GAD activity or a reduction in the levels of free glutamate may prevent the growth of otherwise resistant GAD(+) bacteria in foods where low pH and/or nisin is used as a preservative.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

R Paul Ross, Colin Hill, Paul D. Cotter, Maire Begley

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Paul D. Cotter
Teagasc
Total Publications: 253
 
4
Maire Begley
University College Cork
Total Publications: 27