Type

Journal Article

Authors

C G M Gahan
C Hill
M Begley
S Ryan

Subjects

Microbiology

Topics
sigb protein bacteria bacterial proteins microbiology genetics sigma factor metabolism stress physiological listeria monocytogenes growth development environment gene expression regulation bacterial meat products genes bacterial peptide termination factors prfa protein listeria monocytogenes

A five-gene stress survival islet (SSI-1) that contributes to the growth of Listeria monocytogenes in suboptimal conditions. (2010)

Abstract The aim of this study was to examine the contribution of a five-gene islet (lmo0444 - lmo0448) to the growth of Listeria monocytogenes under suboptimal conditions. Bioinformatics and PCR analyses revealed that a five-gene islet is present in c. half of all L. monocytogenes strains examined (66 in total). A deletion mutant that lacks the entire c. 8ยท7-kb islet was created in L. monocytogenes strain LO28. This mutant was impaired in growth at low pH and at high salt concentrations and demonstrated a decreased ability to survive and grow in a model food system (frankfurters). Transcriptional analysis revealed that the islet is self-regulated in that the product of lmo0445 regulates the expression of the other four genes. A role of the alternative stress sigma factor SigB in regulating the islet was also uncovered. The five-gene islet (herein designated as SSI-1; stress survival islet 1) contributes to the growth of L. monocytogenes under suboptimal conditions. SSI-1 may contribute to the survival of certain strains of L. monocytogenes in food environments.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

C G M Gahan, C Hill, M Begley, S Ryan

Experts in our system

1
Cormac G M Gahan
University College Cork
Total Publications: 109
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Maire Begley
University College Cork
Total Publications: 27