Type

Journal Article

Authors

John R Tagg
R Paul Ross
Paul D. Cotter
Colin Hill
Friedrich Götz
Angela Rossney
Paula M. O'Connor
Ralph W Jack
Philip A Wescombe
Lorraine A Draper
and 3 others

Subjects

Microbiology

Topics
drug effects genetics microbiology peptides mutagenesis staphylococcin methicillin resistant staphylococcus aureus mass spectrometry gallidermin biosynthesis anti bacterial agents staphylococcal infections community acquired infections humans metabolism genetics microbial pharmacology isolation purification bacteriocins chemistry

Production of the Bsa lantibiotic by community-acquired Staphylococcus aureus strains. (2009)

Abstract Lantibiotics are antimicrobial peptides that have been the focus of much attention in recent years with a view to clinical, veterinary, and food applications. Although many lantibiotics are produced by food-grade bacteria or bacteria generally regarded as safe, some lantibiotics are produced by pathogens and, rather than contributing to food safety and/or health, add to the virulence potential of the producing strains. Indeed, genome sequencing has revealed the presence of genes apparently encoding a lantibiotic, designated Bsa (bacteriocin of Staphylococcus aureus), among clinical isolates of S. aureus and those associated with community-acquired methicillin-resistant S. aureus (MRSA) infections in particular. Here, we establish for the first time, through a combination of reverse genetics, mass spectrometry, and mutagenesis, that these genes encode a functional lantibiotic. We also reveal that Bsa is identical to the previously identified bacteriocin staphylococcin Au-26, produced by an S. aureus strain of vaginal origin. Our examination of MRSA isolates that produce the Panton-Valentine leukocidin demonstrates that many community-acquired S. aureus strains, and representatives of ST8 and ST80 in particular, are producers of Bsa. While possession of Bsa immunity genes does not significantly enhance resistance to the related lantibiotic gallidermin, the broad antimicrobial spectrum of Bsa strongly indicates that production of this bacteriocin confers a competitive ecological advantage on community-acquired S. aureus.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

John R Tagg, R Paul Ross, Paul D. Cotter, Colin Hill, Friedrich Götz, Angela Rossney, Paula M. O'Connor, Ralph W Jack, Philip A Wescombe, Lorraine A Draper and 3 others

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Paul D. Cotter
Teagasc
Total Publications: 253
 
3
Colin Hill
University College Cork
Total Publications: 351
 
4
Paula M. O'Connor
Teagasc
Total Publications: 85
 
5
Lorraine A Draper
University College Cork
Total Publications: 24