Type

Journal Article

Authors

Alan D W Dobson
Julian R Marchesi
Fergal O'Gara
Colin Hill
R Paul Ross
Paula M. O'Connor
Mary C. Rea
Marie B Bourke
Marlies J Mooij
Marcella Walsh
and 4 others

Subjects

Microbiology

Topics
base sequence rna ribosomal 16s ireland metabolism phylogeny bacteria antibiosis cluster analysis peptide synthases molecular sequence data animals genetics porifera microbiology anti infective agents sequence analysis dna polyketide synthases species specificity

Isolation and analysis of bacteria with antimicrobial activities from the marine sponge Haliclona simulans collected from Irish waters. (2008)

Abstract Samples of the marine sponge Haliclona simulans were collected from Irish coastal waters, and bacteria were isolated from these samples. Phylogenetic analyses of the cultured isolates showed that four different bacterial phyla were represented; Bacteriodetes, Actinobacteria, Proteobacteria, and Firmicutes. The sponge bacterial isolates were assayed for the production of antimicrobial substances, and biological activities against Gram-positive and Gram-negative bacteria and fungi were demonstrated, with 50% of isolates showing antimicrobial activity against at least one of the test strains. Further testing showed that the antimicrobial activities extended to the important pathogens Pseudomonas aeruginosa, Clostridium difficile, multi-drug-resistant Staphylococcus aureus, and pathogenic yeast strains. The Actinomycetes were numerically the most abundant producers of antimicrobial activities, although activities were also noted from Bacilli and Pseudovibrio isolates. Surveys for the presence of potential antibiotic encoding polyketide synthase and nonribosomal peptide synthetase genes also revealed that genes for the biosynthesis of these secondary metabolites were present in most bacterial phyla but were particularly prevalent among the Actinobacteria and Proteobacteria. This study demonstrates that the culturable fraction of bacteria from the sponge H. simulans is diverse and appears to possess much potential as a source for the discovery of new medically relevant biological active agents.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Alan D W Dobson, Julian R Marchesi, Fergal O'Gara, Colin Hill, R Paul Ross, Paula M. O'Connor, Mary C. Rea, Marie B Bourke, Marlies J Mooij, Marcella Walsh and 4 others

Experts in our system

1
Alan D W Dobson
University College Cork
Total Publications: 98
 
2
Julian R Marchesi
University College Cork
Total Publications: 40
 
3
Fergal O'Gara
University College Cork
Total Publications: 132
 
4
Colin Hill
University College Cork
Total Publications: 351
 
5
R Paul Ross
Teagasc
Total Publications: 441
 
6
Paula M. O'Connor
Teagasc
Total Publications: 85
 
7
Mary C. Rea
Teagasc
Total Publications: 68
 
8
Marlies J Mooij
University College Cork
Total Publications: 23