Type

Journal Article

Authors

Julian R. Marchesi
Yulan Wang
Elaine Holmes
Micheal O'Riordan
Gerald C O'Sullivan
John K Collins
Yvonne Clune
Fergus Shanahan
Pauline D. Scanlan

Subjects

Microbiology

Topics
humans adenomatous polyposis coli female electrophoresis polyacrylamide gel genetics rna ribosomal 16s gastrointestinal tract biodiversity microbiology colorectal neoplasms clostridium classification male intestines dna ribosomal spacer dna bacterial

Culture-independent analysis of the gut microbiota in colorectal cancer and polyposis. (2008)

Abstract A role for the intestinal microbiota is routinely cited as a potential aetiological factor in colorectal cancer initiation and progression. As the majority of bacteria in the gut are refractory to culture we investigated this ecosystem in subjects with colorectal cancer and with adenomatous polyposis who are at high risk of developing colorectal cancer, using culture-independent methods. Twenty colorectal cancer and 20 polypectomized volunteers were chosen for this analysis. An exploration of the diversity and temporal stability of the dominant bacteria and several bacterial subgroups was undertaken using 16S rRNA gene denaturing gradient gel electrophoresis and ribosomal intergenic spacer analysis (RISA). Metabonomic analysis of the distal gut microbiota's environment was also undertaken. A significantly reduced temporal stability and increased diversity for the microbiota of subjects with colorectal cancer and polyposis was evident. A significantly increased diversity of the Clostridium leptum and C. coccoides subgroups was also noted for both disease groups. A clear division in the metabonome was observed for the colorectal cancer and polypectomized subjects compared with control volunteers. The intestinal microbiota and their metabolites are significantly altered in both colorectal cancer and polypectomized subjects compared with controls.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Julian R. Marchesi, Yulan Wang, Elaine Holmes, Micheal O'Riordan, Gerald C O'Sullivan, John K Collins, Yvonne Clune, Fergus Shanahan, Pauline D. Scanlan

Experts in our system

1
Julian R Marchesi
University College Cork
Total Publications: 45
 
2
Elaine Holmes
University College Cork
Total Publications: 7
 
3
Gerald C O'Sullivan
University College Cork
Total Publications: 46
 
4
Fergus Shanahan
University College Cork
Total Publications: 259
 
5
Pauline D. Scanlan
University College Cork
Total Publications: 24