Type

Journal Article

Authors

C Hill
C G M Gahan
E M Ryan

Subjects

Microbiology

Topics
genetics sigma factor metabolism benzalkonium compounds listeria monocytogenes drug resistance bacterial cetylpyridinium growth development sodium dodecyl sulfate pharmacology drug effects gene expression regulation bacterial detergents

A significant role for Sigma B in the detergent stress response of Listeria monocytogenes. (2007)

Abstract To investigate the contribution of the alternative sigma factor Sigma B to detergent stress in Listeria monocytogenes upon exposure to the surface-active agents, benzalkoniumchloride (BC), cetylpyridinium chloride (CPC) and sodium docecyl sulfate (SDS). Surfactant/detergent stress in L. monocytogenes 10403S and a DeltasigB mutant of 10403S was investigated by lethality, impact on growth and by transcriptional analysis. We observed a significant 1-2 log decrease in the viability of the DeltasigB mutant in response to lethal levels of surfactants. Transcriptional (reverse transcriptase-PCR) analysis revealed the induction of sigmaB by sublethal levels of surface-active agents. However, Sigma B does not play a significant role in the growth of L. monocytogenes upon exposure to sublethal levels as investigated by growth analysis. Sigma B is essential for the resistance of L. monocytogenes at lethal levels of BC, CPC and SDS. We demonstrate that Sigma B is essential for the resistance of the pathogen to surfactant stress. The findings raise the possibility that induction of Sigma B by sublethal levels of industrial cleaning agents may promote enhanced resistance of further food-processing associated stresses or conditions encountered during infection. Sigma B-regulated mechanisms of detergent resistance may provide targets for the future design of novel cleaning agents.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

C Hill, C G M Gahan, E M Ryan

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
Cormac G M Gahan
University College Cork
Total Publications: 109