Type

Journal Article

Authors

Fergal O'Gara
Antonio Oliver
Cormac G M Gahan
Claire Adams
Colin Hill
Pat G. Casey
Cristina Gómez
Nuria Borrell
María D Maciá
Eoin P O'Grady
and 2 others

Subjects

Microbiology

Topics
physiology pseudomonas aeruginosa female genetics microbiology animals pseudomonas infections pneumonia pathology mice gene deletion transcription factors pathogenicity virulence factors mice inbred balb c virulence

Pseudomonas aeruginosa RsmA plays an important role during murine infection by influencing colonization, virulence, persistence, and pulmonary inflammation. (2007)

Abstract The ability of Pseudomonas aeruginosa to cause a broad range of infections in humans is due, at least in part, to its adaptability and its capacity to regulate the expression of key virulence genes in response to specific environmental conditions. Multiple two-component response regulators have been shown to facilitate rapid responses to these environmental conditions, including the coordinated expression of specific virulence determinants. RsmA is a posttranscriptional regulatory protein which controls the expression of a number of virulence-related genes with relevance for acute and chronic infections. Many membrane-bound sensors, including RetS, LadS, and GacS, are responsible for the reciprocal regulation of genes associated with acute infection and chronic persistence. In P. aeruginosa this is due to sensors influencing the expression of the regulatory RNA RsmZ, with subsequent effects on the level of free RsmA. While interactions between an rsmA mutant and human airway epithelial cells have been examined in vitro, the role of RsmA during infection in vivo has not been determined yet. Here the function of RsmA in both acute and chronic models of infection was examined. The results demonstrate that RsmA is involved in initial colonization and dissemination in a mouse model of acute pneumonia. Furthermore, while loss of RsmA results in reduced colonization during the initial stages of acute infection, the data show that mutation of rsmA ultimately favors chronic persistence and results in increased inflammation in the lungs of infected mice.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Fergal O'Gara, Antonio Oliver, Cormac G M Gahan, Claire Adams, Colin Hill, Pat G. Casey, Cristina Gómez, Nuria Borrell, María D Maciá, Eoin P O'Grady and 2 others

Experts in our system

1
Fergal O'Gara
University College Cork
Total Publications: 132
 
2
Cormac G M Gahan
University College Cork
Total Publications: 109
 
3
Claire Adams
University College Cork
Total Publications: 27
 
4
Colin Hill
University College Cork
Total Publications: 351
 
5
Pat G. Casey
University College Cork
Total Publications: 40