Journal Article


Gerald F Fitzgerald
Colin Hill
Roy D. Sleator
Vivien M Sheehan



gastrointestinal transit microbial viability microbiology animals gastric acid listeriosis mice anti bacterial agents administration dosage colony count microbial intestines carrier proteins bacterial proteins betl protein listeria monocytogenes administration oral prevention control genetics listeria monocytogenes mice inbred balb c biosynthesis pharmacokinetics growth development cecum probiotics physiology pharmacology feces bifidobacterium

Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium breve UCC2003. (2007)

Abstract Given the increasing commercial and clinical relevance of probiotic cultures, improving their stress tolerance profile and ability to overcome the physiological defences of the host is an important biological goal. In order to reach the gastrointestinal tract in sufficient numbers to exert a therapeutic effect, probiotic bacteria must resist the deleterious actions of low pH, elevated osmolarity and bile salts. Cloning the listerial betaine uptake system, BetL, into the probiotic strain Bifidobacterium breve UCC2003 significantly improved probiotic tolerance to gastric juice and conditions of elevated osmolarity mimicking the gut environment. Furthermore, whilst stable colonization of the murine intestine was achieved by oral administration of B. breve UCC2003, strains harbouring BetL were recovered at significantly higher levels in the faeces, intestines and caecum of inoculated animals. Finally, in addition to improved gastric transit and intestinal persistence, this approach improved the clinical efficacy of the probiotic culture: mice fed B. breve UCC2003-BetL(+) exhibited significantly lower levels of systemic infection compared to the control strain following oral inoculation with Listeria monocytogenes.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Gerald F Fitzgerald, Colin Hill, Roy D. Sleator, Vivien M Sheehan

Experts in our system

Gerald F Fitzgerald
Total Publications: 207
Colin Hill
University College Cork
Total Publications: 351
Roy D. Sleator
University College Cork
Total Publications: 44