Type

Journal Article

Authors

Colin Hill
Cormac G M Gahan
Helena M Stack

Subjects

Microbiology

Topics
genetics listeria monocytogenes gene expression regulation bacterial microbiology hydrogen ion concentration pathogenicity virulence mice promoter regions genetic macrophages physiology plasmids phagosomes animals chemistry

A novel promoter trap identifies Listeria monocytogenes promoters expressed at a low pH within the macrophage phagosome. (2007)

Abstract The Gram-positive pathogen Listeria monocytogenes encounters acid environments in low-pH foods, during passage through the stomach and within the macrophage phagosome during systemic infection. A novel promoter-trap system termed pGAD-HLY was developed, based on a plasmid containing a promoterless copy of gadB (required for survival at low pH) and hly (whose product facilitates escape from the macrophage phagosome) to identify loci that are induced under different stress conditions in vitro as well as identifying in vivo inducible promoters expressed during intracellular infection. This system facilitated the identification of 11 acid-inducible genes in L. monocytogenes. Transcriptional analysis and acid tolerance response assays confirmed the low-pH induction of these loci, validating this promoter-trap system. Macrophage assays revealed the phagosomal induction of three clones, corresponding to lmo0095, lmo2565 and lmo2371, with two of these clones (lmo0095 and lmo2565) also being induced during murine infection. However, virulence studies did not show any significant difference between strains carrying insertional mutations in these genes and the wild type strain. Although the loci that were identified by this screening procedure do not appear to be central to listerial pathogenesis, it is evident from studies that they contribute to the 'fitness' of this pathogen in adverse acid conditions.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Colin Hill, Cormac G M Gahan, Helena M Stack

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
Cormac G M Gahan
University College Cork
Total Publications: 109