Type

Journal Article

Authors

R Paul Ross
Colin Hill
Paul D. Cotter
Elaine M. Lawton

Subjects

Microbiology

Topics
antibiosis chemistry genetics isolation purification metabolism amino acid sequence bacillus multigene family genome bacterial bacteriocins mass spectrometry biosynthesis gram positive bacteria computational biology genes bacterial pharmacology molecular sequence data microbial sensitivity tests anti bacterial agents growth development gene order drug effects mutagenesis site directed haloduracin bacillus halodurans peptides

Identification of a novel two-peptide lantibiotic, haloduracin, produced by the alkaliphile Bacillus halodurans C-125. (2007)

Abstract Complete genome sequencing of the alkaliphilic bacterium Bacillus halodurans C-125 revealed the presence of several genes homologous to those involved in the production of lantibiotic peptides. Additional bioinformatic analysis identified a total of eleven genes, spanning a 15 kbp region, potentially involved in the production, modification, immunity and transport of a two-peptide lantibiotic. Having established that strain C-125 exhibited antimicrobial activity against a wide range of Gram-positive bacteria, it was demonstrated through peptide purification, MS and site-directed mutagenesis that this activity was indeed attributable to the production of a lantibiotic encoded by these genes. This antimicrobial has been designated haloduracin and represents the first occasion wherein production of two-peptide lantibiotic has been associated with a Bacillus sp. It is also the first example of a lantibiotic of any kind to be produced by an alkaliphilic species.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

R Paul Ross, Colin Hill, Paul D. Cotter, Elaine M. Lawton

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Paul D. Cotter
Teagasc
Total Publications: 253
 
4
Elaine M. Lawton
University College Cork
Total Publications: 12