Type

Journal Article

Authors

Julian R Marchesi
Brian V Jones

Subjects

Microbiology

Topics
metabolism genome bacterial humans dna transposable elements escherichia coli molecular sequence data genetics dna bacterial gastrointestinal tract microbiology gene transfer techniques plasmids sequence analysis dna

Transposon-aided capture (TRACA) of plasmids resident in the human gut mobile metagenome. (2006)

Abstract The bacterial community of the human gut is a complex ecosystem composed of >1,000 species, the majority of which are Gram positive and uncultured. To study plasmids resident within this community, we developed a culture-independent transposon aided capture method (TRACA), which does not rely on any plasmid-encoded traits. TRACA facilitated acquisition of plasmids from metagenomic DNA extracts, and subsequent maintenance and selection in an Escherichia coli host. We confirmed the presence of the transposon in captured plasmids and demonstrate that these plasmids are mainly of a Gram-positive origin. Sequencing of plasmids designated pTRACA10 (7 kb) and pTRACA17 (2.7 kb) revealed genes involved in plasmid mobilization and replication. From the homologies of these genes we conclude that pTRACA17 originates from a Gram-positive host belonging to the Firmicute division. pTRACA10 had two additional open reading frames with similarity to a conserved hypothetical protein and phosphoesterase or phosphohydrolase enzymes (Clusters of Orthologous Groups number 4186). Both plasmids lacked any conventional selectable markers.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Julian R Marchesi, Brian V Jones

Experts in our system

1
Julian R Marchesi
University College Cork
Total Publications: 45
 
2
Brian V Jones
University College Cork
Total Publications: 8