Type

Journal Article

Authors

R Paul Ross
Colin Hill
Olivia McAuliffe
Elaine M. Lawton
Lorraine A Draper
Paul D. Cotter

Subjects

Biochemistry

Topics
biomedical engineering genetics lacticin 481 metabolism dna primers base sequence biosynthesis lactococcus bacterial proteins bacteriocins

Overproduction of wild-type and bioengineered derivatives of the lantibiotic lacticin 3147. (2006)

Abstract Lacticin 3147 is a broad-spectrum two-peptide lantibiotic whose genetic determinants are located on two divergent operons on the lactococcal plasmid pMRC01. Here we introduce each of 14 subclones, containing different combinations of lacticin 3147 genes, into MG1363 (pMRC01) and determine that a number of them can facilitate overproduction of the lantibiotic. Based on these studies it is apparent that while the provision of additional copies of genes encoding the biosynthetic/production machinery and the regulator LtnR is a requirement for high-level overproduction, the presence of additional copies of the structural genes (i.e., ltnA1A2) is not.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

R Paul Ross, Colin Hill, Olivia McAuliffe, Elaine M. Lawton, Lorraine A Draper, Paul D. Cotter

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Olivia McAuliffe
Teagasc
Total Publications: 64
 
4
Elaine M. Lawton
University College Cork
Total Publications: 12
 
5
Lorraine A Draper
University College Cork
Total Publications: 24
 
6
Paul D. Cotter
Teagasc
Total Publications: 253