Type

Journal Article

Authors

R Paul Ross
Aidan Coffey
Gerald F Fitzgerald
Rob Edwards
Michael Callanan
Olivia McAuliffe
Maeve Trotter

Subjects

Microbiology

Topics
genetics genome viral sequence homology nucleic acid open reading frames classification viral proteins bacteriophages sequence analysis dna lactococcus lactis endonucleases dna modification methylases virology

Genome analysis of the obligately lytic bacteriophage 4268 of Lactococcus lactis provides insight into its adaptable nature. (2005)

Abstract Analysis of the complete nucleotide sequence of the lactococcal phage 4268, which is lytic for the cheese starter Lactococcus lactis DPC4268, is presented. Phage 4268 has a linear genome of 36,596 bp, which is modularly organised and encompasses 49 open reading frames. Putative functions were assigned to approximately 45% of the predicted products of these open reading frames based on sequence similarity with known proteins, N-terminal sequence analysis and identification of conserved domains. Significantly, a segment of the genome has homology to the recently sequenced lysogenic module in lactococcal phage phi31 that contains a lytic switch but no phage integrase or attachment site. This suggests that it is derived from a prophage. A phage 4268-encoded and a host-encoded methylase were found to be highly similar, having only two nucleotide mismatches, suggesting that the phage acquired the methylase gene to protect it from a host endonuclease. Comparative genomic analysis revealed significant homology between phage 4268 and the lactococcal phage BK5-T. The comparative analysis also supported the classification of phage 4268 and other BK5-T-related phage as separate from the proposed P335 species of lactococcal phage.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

R Paul Ross, Aidan Coffey, Gerald F Fitzgerald, Rob Edwards, Michael Callanan, Olivia McAuliffe, Maeve Trotter

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 452
 
2
Aidan Coffey
Teagasc
Total Publications: 66
 
3
Gerald F Fitzgerald
Teagasc
Total Publications: 208
 
4
Olivia McAuliffe
Teagasc
Total Publications: 69