Journal Article


Cormac G M Gahan
Colin Hill
Megan Bowers
Roy D. Sleator
Helena M Stack



bacterial proteins chemistry amino acid sequence genetics metabolism listeria monocytogenes virulence penicillin g heat shock response molecular sequence data pathogenicity physiology mice inbred balb c listeriosis pharmacology sequence analysis dna hot temperature enzymology animals microbiology serine endopeptidases mice gene expression regulation bacterial heat shock proteins hydrogen ion concentration

Role for HtrA in stress induction and virulence potential in Listeria monocytogenes. (2005)

Abstract In silico analysis of the Listeria monocytogenes genome revealed lmo0292, a gene predicted to encode a HtrA-like serine protease. A stable insertion mutant was constructed, revealing a requirement for htrA in the listerial response to heat, acid, and penicillin stress. Transcriptional analysis revealed that htrA is not induced in response to heat shock but is induced in response to low pH and penicillin G stress. Furthermore, htrA expression was shown to be dependent upon the LisRK two-component sensor-kinase, a system known to respond to changes in integrity of the cell envelope. In addition, we demonstrated that a second in-frame start codon, upstream of that previously annotated for L. monocytogenes htrA, incorporating a putative signal sequence appears to influence virulence potential. Finally, a significant virulence defect was observed for the htrA mutant, indicating that this gene is required for full virulence in mice. Our findings suggest that L. monocytogenes lmo0292 encodes an HtrA-like serine protease that is not part of the classical heat shock response but is involved in stress responses and virulence.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Cormac G M Gahan, Colin Hill, Megan Bowers, Roy D. Sleator, Helena M Stack

Experts in our system

Cormac G M Gahan
University College Cork
Total Publications: 109
Colin Hill
University College Cork
Total Publications: 351
Roy D. Sleator
University College Cork
Total Publications: 44