Type

Journal Article

Authors

Matthias Eberl
Hassan Jomaa
Colin Hill
Martin Hintz
Ann-Kristin Kollas
Cormac G M Gahan
Maire Begley

Subjects

Microbiology

Topics
immunology mutation diphosphates genetics listeria monocytogenes mevalonic acid microbiology lymphocyte activation sugar phosphates receptors antigen t cell gamma delta 2 c methylerythritol 4 phosphate erythritol metabolism humans t lymphocytes analogs derivatives terpenes 4 hydroxy 3 methylbut 2 enyl pyrophosphate

The interplay between classical and alternative isoprenoid biosynthesis controls gammadelta T cell bioactivity of Listeria monocytogenes. (2004)

Abstract Isoprenoids are synthesised either through the classical, mevalonate pathway, or the alternative, non-mevalonate, 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. The latter is found in many microbial pathogens and proceeds via (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP), a potent activator of human Vgamma9/Vdelta2 T cells. Listeria monocytogenes is the only pathogenic bacterium known to contain both pathways concurrently. Strategic gene knockouts demonstrate that either pathway is functional but dispensable for viability. Yet, disrupting the mevalonate pathway results in a complementary upregulation of the MEP pathway. Vgamma9/Vdelta2 T cell bioactivity is increased in DeltalytB mutants where HMB-PP accumulation is expected, and lost in DeltagcpE mutants which fail to produce HMB-PP.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Matthias Eberl, Hassan Jomaa, Colin Hill, Martin Hintz, Ann-Kristin Kollas, Cormac G M Gahan, Maire Begley

Experts in our system

1
Matthias Eberl
University College Cork
Total Publications: 4
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Cormac G M Gahan
University College Cork
Total Publications: 109
 
4
Maire Begley
University College Cork
Total Publications: 27