Type

Journal Article

Authors

R Paul Ross
Colin Hill
Paul D. Cotter

Subjects

Biochemistry

Topics
trans activators recombination genetic replication initiator protein metabolism plasmids proteins lactococcus lactis bacterial proteins conjugation genetic genetics food industry genetic vectors food microbiology dna binding proteins dna helicases genetic engineering

A food-grade approach for functional analysis and modification of native plasmids in Lactococcus lactis. (2003)

Abstract While plasmids from lactic acid bacteria possess many traits that are of industrial value, their exploitation is often frustrated by an inability to conduct food-grade engineering of native plasmids or to readily screen for their transfer. Here we describe a system that uses a RepA(+) temperature-sensitive helper plasmid and a RepA(-) cloning vector to overcome these problems while maintaining the food-grade status of the native plasmid. This strategy was used to precisely delete ltnA1 alone, or in conjunction with ltnA2 (encoding the structural proteins of the lantibiotic lacticin 3147), from the native 60.2-kb plasmid pMRC01 and to select for the transfer of pMRC01 between Lactococcus lactis strains.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

R Paul Ross, Colin Hill, Paul D. Cotter

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Paul D. Cotter
Teagasc
Total Publications: 253