Journal Article


Colin Hill
Caitriona M Guinane
Paul D. Cotter



genetics microbial sensitivity tests anti bacterial agents signal transduction listeria monocytogenes plasmids protein kinases reverse transcriptase polymerase chain reaction nisin protein histidine kinase culture media cephalosporins physiology pharmacology drug effects gene expression regulation bacterial transcription factors

The LisRK signal transduction system determines the sensitivity of Listeria monocytogenes to nisin and cephalosporins. (2002)

Abstract The Listeria monocytogenes two-component signal transduction system, LisRK, initially identified in strain LO28, plays a significant role in the virulence potential of this important food-borne pathogen. Here, it is shown that, in addition to its major contribution in responding to ethanol, pH, and hydrogen peroxide stresses, LisRK is involved in the ability of the cell to tolerate important antimicrobials used in food and in medicine, e.g., the lantibiotic nisin and the cephalosporin family of antibiotics. A (Delta)lisK mutant (lacking the LisK histidine kinase sensor component) displays significantly enhanced resistance to the lantibiotic nisin, a greatly enhanced sensitivity to the cephalosporins, and a large reduction in the expression of three genes thought to encode a penicillin-binding protein, another histidine kinase (other than LisK), and a protein of unknown function. Confirmation of the role of LisRK was obtained when the response regulator, LisR, was overexpressed using both constitutive and inducible (nisin-controlled expression) systems. Under these conditions we observed a reversion of the (Delta)lisK mutant to wild-type growth kinetics in the presence of nisin. It was also found that overexpression of LisR complemented the reduced expression of two of the aforementioned genes. These results demonstrate the important role of LisRK in the response of L. monocytogenes to a number of antimicrobial agents.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Colin Hill, Caitriona M Guinane, Paul D. Cotter

Experts in our system

Colin Hill
University College Cork
Total Publications: 351
Caitriona M Guinane
Total Publications: 35
Paul D. Cotter
Total Publications: 253