Type

Journal Article

Authors

C Hill
B O'Driscoll
R D Sleator

Subjects

Biochemistry

Topics
mutagenesis mice listeria monocytogenes gene expression regulation bacterial carrier proteins growth development cheese pathogenicity mice inbred balb c rna bacterial virulence transcription genetic physiology bacterial proteins metabolism sodium chloride betaine osmotic pressure animals betl protein listeria monocytogenes microbiology genetics

Analysis of the role of betL in contributing to the growth and survival of Listeria monocytogenes LO28. (2000)

Abstract Survival of the food-borne pathogen Listeria monocytogenes in environments of elevated osmolarity and reduced temperature is attributed, at least in part, to the accumulation of the trimethylammonium compound glycine betaine. Previously we identified betL, a gene encoding the secondary glycine betaine transporter BetL, which we linked to the salt tolerance of Listeria. In this report, we demonstrate that betL, preceded by a consensus sigmaB-dependent promoter, is regulated by osmotic up-shock, at least in part at the level of transcription. Using allelic exchange mutagenesis we constructed an in-frame deletion in betL, and used this mutant to determine the role of BetL in contributing to the growth and survival of L. monocytogenes, both in a high risk food (Camembert cheese) and animal model. Our results indicate that while BetL plays an important role in glycine betaine mediated osmoprotection, mutating the gene does not significantly effect either the cryotolerance or virulence of the organism.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

C Hill, B O'Driscoll, R D Sleator

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
Roy D. Sleator
University College Cork
Total Publications: 44