Type

Journal Article

Authors

R O'Connor
P T Walsh

Subjects

Biochemistry

Topics
antagonists inhibitors genetics immunology signal transduction lymphocyte activation cell survival proto oncogene proteins c bcl 2 receptor igf type 1 physiology pharmacology interleukin 2 fluorescent antibody technique bcl x protein blotting western antigens cd3 apoptosis receptor aggregation bcl2l1 protein human antigens cd80 receptors antigen t cell antigens cd95 cells cultured insulin like growth factor i metabolism humans flow cytometry antibodies jurkat cells cytology drug effects t lymphocytes antigens cd28

The insulin-like growth factor-I receptor is regulated by CD28 and protects activated T cells from apoptosis. (2000)

Abstract Co-stimulatory signals through the CD28 receptor enhance the survival of T cells that have their antigen receptor (TCR) engaged. Here we show that stimulation through the CD28 receptor in the absence of TCR engagement with either an anti-CD28 cross-linking antibody or the CD80 ligand transiently increases expression of the insulin-like growth factor-I receptor (IGF-IR) on T cells. Antibodies that block signaling through the IGF-IR decrease the survival of T cells activated through the TCR and CD28 in the presence of IL-2 by more than 50%, and also enhance susceptibility to Fas-induced apoptosis. CD28 stimulation increases IGF-IR expression on Jurkat cells, and exogenously added IGF-I can protect these cells from Fas-induced apoptosis. We conclude that CD28-mediated enhancement of IGF-IR expression provides activated T cells with essential survival signals that are independent of survival mediated by IL-2 and Bcl-xl.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

R O'Connor, P T Walsh

Experts in our system

1
Robert O'Connor
Dublin City University
Total Publications: 74