Type

Journal Article

Authors

F O'Gara
D N Dowling
D J O'Sullivan
M J Callanan
P R Gill
R Sexton

Subjects

Microbiology

Topics
trans activators pharmacology base sequence physiology phenotype gene expression regulation bacterial escherichia coli mutagenesis insertional pseudobactin receptors cell surface promoter regions genetic pseudobactin receptor pseudomonas iron bacterial proteins drug effects recombinant fusion proteins pseudomonas fluorescens genetics molecular sequence data amino acid sequence oligopeptides sequence homology genes bacterial transcription genetic pbra protein pseudomonas fluorescens sequence alignment biosynthesis

Iron-responsive gene expression in Pseudomonas fluorescens M114: cloning and characterization of a transcription-activating factor, PbrA. (1995)

Abstract In response to iron limitation. Pseudomonas fluorescens M114 induces a number of genes including an iron-scavenging siderophore termed pseudobactin M114, its cognate receptor, PbuA, and a casein protease. A Tn5lacZ-induced mutant (M114FA1) was isolated that exhibits a pleiotropic phenotype and lacks the ability to express these iron-regulated genes. A cosmid clone was identified which complements this mutation. This clone is capable of activating a number of iron-regulated promoter fusion constructs from P. fluorescens M114 and Pseudomonas putida WCS358 and can also promote expression of these fusions in Escherichia coli. A series of insertion mutants was constructed by homologous recombination which were unable to transcribe the promoter fusions. DNA sequence analysis of the complementing region identified one open reading frame (ORF) termed pbrA (pseudobactin regulation activation) and the deduced amino acid sequence shows domains with significant homology to a number of ECF (extracytoplasmic function) transcriptional regulators of the sigma 70 sigma factor family, including fecl required for expression of the ferric dicitrate outer-membrane receptor protein of E. coli. Sequences upstream of the pbrA gene suggest that transcription of pbrA may also be iron regulated.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

F O'Gara, D N Dowling, D J O'Sullivan, M J Callanan, P R Gill, R Sexton

Experts in our system

1
Fergal O'Gara
University College Cork
Total Publications: 132
 
2
D J O'Sullivan
University College Cork
Total Publications: 3
 
3
Michael Callanan
Teagasc
Total Publications: 11