Journal Article


Dimitrios Rizos
Patrick Lonergan
Alfonso Gutierrez-Adan
Thomas E Spencer
Gregory W Burns
Vitezslav Havlicek
Urban Besenfelder
Niamh Forde
Peadar Ó Gaora
Veronica Maillo



traffic differentially expressed genes embryo transfer microarray analysis detection cattle corpus luteum epithelial cells

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street? (2015)

Abstract This study examined the effect of the presence of single or multiple embryos on the transcriptome of the bovine oviduct. In experiment 1, cyclic (nonbred, n = 6) and pregnant (artificially inseminated, n = 11) heifers were slaughtered on Day 3 after estrus, and the ampulla and isthmic regions of the oviduct ipsilateral to the corpus luteum were separately flushed. Oviductal epithelial cells from the isthmus region, in which all oocytes/embryos were located, were snap-frozen for microarray analysis. In experiment 2, heifers were divided into cyclic (nonbred, n = 6) or pregnant (multiple embryo transfer, n = 10) groups. In vitro-produced presumptive zygotes were transferred endoscopically to the ipsilateral oviduct on Day 1.5 postestrus (n = 50 zygotes/heifer). Heifers were slaughtered on Day 3, and oviductal isthmus epithelial cells were recovered for RNA sequencing. Microarray analysis in experiment 1 failed to detect any difference in the transcriptome of the oviductal isthmus induced by the presence of a single embryo. In experiment 2, following multiple embryo transfer, RNA sequencing revealed 278 differentially expressed genes, of which 123 were up-regulated and 155 were down-regulated in pregnant heifers. Most of the down-regulated genes were related to immune function. In conclusion, the presence of multiple embryos in the oviduct resulted in the detection of differentially expressed genes in the oviductal isthmus; failure to detect changes in the oviduct transcriptome in the presence of a single embryo may be due to the effect being local and undetectable under the conditions of this study.
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Full list of authors on original publication

Dimitrios Rizos, Patrick Lonergan, Alfonso Gutierrez-Adan, Thomas E Spencer, Gregory W Burns, Vitezslav Havlicek, Urban Besenfelder, Niamh Forde, Peadar Ó Gaora, Veronica Maillo

Experts in our system

D Rizos
University College Dublin
Total Publications: 42
P. Lonergan
University College Dublin
Total Publications: 207
Alfonso Gutierrez-Adan
University College Dublin
Total Publications: 9
Thomas E Spencer
University College Dublin
Total Publications: 14
N Forde
University College Dublin
Total Publications: 55
V Maillo
University College Dublin
Total Publications: 8