Journal Article


Sean P. Colgan
Cormac T. Taylor
Vu Nguyen
Jordi M Lanis
Kristine A Kuhn
Christina Pickel
Stefan F Ehrentraut
Tiffany L Weir
Aaron Magnuson
Douglas J Kominsky
and 8 others



fatty acids fatty acid gut microbiota barrier function intestinal microbiota hypoxia inducible factor hif transcription factor intestinal function

Crosstalk between Microbiota-Derived Short-Chain Fatty Acids and Intestinal Epithelial HIF Augments Tissue Barrier Function. (2014)

Abstract Interactions between the microbiota and distal gut are fundamental determinants of human health. Such interactions are concentrated at the colonic mucosa and provide energy for the host epithelium through the production of the short-chain fatty acid butyrate. We sought to determine the role of epithelial butyrate metabolism in establishing the austere oxygenation profile of the distal gut. Bacteria-derived butyrate affects epithelial O2 consumption and results in stabilization of hypoxia-inducible factor (HIF), a transcription factor coordinating barrier protection. Antibiotic-mediated depletion of the microbiota reduces colonic butyrate and HIF expression, both of which are restored by butyrate supplementation. Additionally, germ-free mice exhibit diminished retention of O2-sensitive dyes and decreased stabilized HIF. Furthermore, the influences of butyrate are lost in cells lacking HIF, thus linking butyrate metabolism to stabilized HIF and barrier function. This work highlights a mechanism where host-microbe interactions augment barrier function in the distal gut.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Sean P. Colgan, Cormac T. Taylor, Vu Nguyen, Jordi M Lanis, Kristine A Kuhn, Christina Pickel, Stefan F Ehrentraut, Tiffany L Weir, Aaron Magnuson, Douglas J Kominsky and 8 others

Experts in our system

Sean P. Colgan
University College Dublin
Total Publications: 7
Cormac T. Taylor
University College Dublin
Total Publications: 110
Christina Pickel
University College Dublin
Total Publications: 4