Type

Journal Article

Authors

Marco P. Monopoli
Yan Yan
Kenneth A. Dawson
Frank Caruso
Pauline M Rudd
Henning Stöckmann
Eugene Mahon
Philip M Kelly
Sha Wan

Subjects

Biochemistry

Topics
protein corona protein glycosylation pro inflammatory nanoparticle cell membrane cell adhesion cell side

The "sweet" side of the protein corona: effects of glycosylation on nanoparticle-cell interactions. (2015)

Abstract The significance of a protein corona on nanoparticles in modulating particle properties and their biological interactions has been widely acknowledged. The protein corona is derived from proteins in biological fluids, many of which are glycosylated. To date, the glycans on the proteins have been largely overlooked in studies of nanoparticle-cell interactions. In this study, we demonstrate that glycosylation of the protein corona plays an important role in maintaining the colloidal stability of nanoparticles and influences nanoparticle-cell interactions. The removal of glycans from the protein corona enhances cell membrane adhesion and cell uptake of nanoparticles in comparison with the fully glycosylated form, resulting in the generation of a pro-inflammatory milieu by macrophages. This study highlights that the post-translational modification of proteins can significantly impact nanoparticle-cell interactions by modulating the protein corona properties.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Marco P. Monopoli, Yan Yan, Kenneth A. Dawson, Frank Caruso, Pauline M Rudd, Henning Stöckmann, Eugene Mahon, Philip M Kelly, Sha Wan

Experts in our system

1
Marco P. Monopoli
University College Dublin
Total Publications: 34
 
2
Kenneth A. Dawson
University College Dublin
Total Publications: 152
 
3
Pauline M Rudd
University College Dublin
Total Publications: 45
 
4
Eugene Mahon
University College Dublin
Total Publications: 12