Type

Journal Article

Authors

Karsten Hokamp
David E. MacHugh
Stephen V. Gordon
Eamonn Gormley
Nicolas C. Nalpas
Kevin M. Conlon
John A Browne
Maria Taraktsoglou
Stephen D. E. Park
David A. Magee
and 1 others

Subjects

Veterinary

Topics
cattle cell death networks environment macrophage genes immune responses mycobacterium bovis

Key Hub and Bottleneck Genes Differentiate the Macrophage Response to Virulent and Attenuated Mycobacterium bovis. (2014)

Abstract Mycobacterium bovis is an intracellular pathogen that causes tuberculosis in cattle. Following infection, the pathogen resides and persists inside host macrophages by subverting host immune responses via a diverse range of mechanisms. Here, a high-density bovine microarray platform was used to examine the bovine monocyte-derived macrophage transcriptome response to M. bovis infection relative to infection with the attenuated vaccine strain, M. bovis Bacille Calmette-Guérin. Differentially expressed genes were identified (adjusted P-value ≤0.01) and interaction networks generated across an infection time course of 2, 6, and 24 h. The largest number of biological interactions was observed in the 24-h network, which exhibited scale-free network properties. The 24-h network featured a small number of key hub and bottleneck gene nodes, including IKBKE, MYC, NFKB1, and EGR1 that differentiated the macrophage response to virulent and attenuated M. bovis strains, possibly via the modulation of host cell death mechanisms. These hub and bottleneck genes represent possible targets for immuno-modulation of host macrophages by virulent mycobacterial species that enable their survival within a hostile environment.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Karsten Hokamp, David E. MacHugh, Stephen V. Gordon, Eamonn Gormley, Nicolas C. Nalpas, Kevin M. Conlon, John A Browne, Maria Taraktsoglou, Stephen D. E. Park, David A. Magee and 1 others

Experts in our system

1
Karsten Hokamp
Trinity College Dublin
Total Publications: 47
 
2
David E. MacHugh
University College Dublin
Total Publications: 82
 
3
Stephen V. Gordon
University College Dublin
Total Publications: 40
 
4
E Gormley
University College Dublin
Total Publications: 68
 
5
Nicolas C. Nalpas
University College Dublin
Total Publications: 14
 
6
John A Browne
University College Dublin
Total Publications: 73
 
7
Stephen D. E. Park
University College Dublin
Total Publications: 35
 
8
David A. Magee
University College Dublin
Total Publications: 47