Type

Journal Article

Authors

Kevin O'Connor
Ramesh P Babu
Trevor Woods
Maciej W Guzik
Shane T Kenny
Ian Belton
Reeta Davis
Eoin Casey
Gearoid Duane
Federico Cerrone

Subjects

Chemistry

Topics
oxygen consumption chain length growth rate cultivation h 2 butyric acid pseudomonas putida cell density

Fed-batch strategies using butyrate for high cell density cultivation of Pseudomonas putida and its use as a biocatalyst. (2014)

Abstract A mathematically based fed-batch bioprocess demonstrated the suitability of using a relatively cheap and renewable substrate (butyric acid) for Pseudomonas putida CA-3 high cell density cultivation. Butyric acid fine-tuned addition is critical to extend the fermentation run and avoid oxygen consumption while maximising the biomass volumetric productivity. A conservative submaximal growth rate (μ of 0.25 h(-1)) achieved 71.3 g L(-1) of biomass after 42 h of fed-batch growth. When a more ambitious feed rate was supplied in order to match a μ of 0.35 h(-1), the volumetric productivity was increased to 2.0 g L(-1) h(-1), corresponding to a run of 25 h and 50 g L(-1) of biomass. Both results represent the highest biomass and the best biomass volumetric productivity with butyrate as a sole carbon source. However, medium chain length polyhydroxyalkanoate (mcl-PHA) accumulation with butyrate grown cells is low (4 %). To achieve a higher mcl-PHA volumetric productivity, decanoate was supplied to butyrate grown cells. This strategy resulted in a PHA volumetric productivity of 4.57 g L(-1) h(-1) in the PHA production phase and 1.63 g L(-1) h(-1)over the lifetime of the fermentation, with a maximum mcl-PHA accumulation of 65 % of the cell dry weight.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Kevin O'Connor, Ramesh P Babu, Trevor Woods, Maciej W Guzik, Shane T Kenny, Ian Belton, Reeta Davis, Eoin Casey, Gearoid Duane, Federico Cerrone

Experts in our system

1
Kevin E O'Connor
University College Dublin
Total Publications: 52
 
2
Ramesh P Babu
University College Dublin
Total Publications: 11
 
3
Trevor Woods
Trinity College Dublin
Total Publications: 11
 
4
Shane T Kenny
University College Dublin
Total Publications: 15
 
5
Eoin Casey
University College Dublin
Total Publications: 68