Type

Journal Article

Authors

Eamonn Gormley
Leigh A L Corner
Tara Fitzsimons
Mark A Chambers
Sandrine Lesellier
Frank E Aldwell
Eamon Costello
Denise Murphy

Subjects

Microbiology

Topics
metabolism tuberculosis mustelidae vaccination interferon gamma bcg vaccine animals immunology prevention control lymph nodes microbiology administration oral lung standards veterinary mycobacterium bovis

Oral vaccination of badgers (Meles meles) against tuberculosis: comparison of the protection generated by BCG vaccine strains Pasteur and Danish. (2013)

Abstract Vaccination of badgers by the subcutaneous, mucosal and oral routes with the Pasteur strain of Mycobacterium bovis bacille Calmette-Guérin (BCG) has resulted in significant protection against experimental infection with virulent M. bovis. However, as the BCG Danish strain is the only commercially licensed BCG vaccine for use in humans in the European Union it is the vaccine of choice for delivery to badger populations. As all oral vaccination studies in badgers were previously conducted using the BCG Pasteur strain, this study compared protection in badgers following oral vaccination with the Pasteur and the Danish strains. Groups of badgers were vaccinated orally with 10(8) colony forming units (CFU) BCG Danish 1331 (n = 7 badgers) or 10(8) CFU BCG Pasteur 1173P2 (n = 6). Another group (n = 8) served as non-vaccinated controls. At 12 weeks post-vaccination, the animals were challenged by the endobronchial route with 6 × 10(3) CFU M. bovis, and at 15 weeks post-infection, all of the badgers were euthanased. Vaccination with either BCG strain provided protection against challenge compared with controls. The vaccinated badgers had significantly fewer sites with gross pathology and significantly lower gross pathological severity scores, fewer sites with histological lesions and fewer sites of infection, significantly lower bacterial counts in the thoracic lymph node, and lower bacterial counts in the lungs than the control group. No differences were observed between either of the vaccine groups by any of the pathology and bacteriology measures. The ELISPOT analysis, measuring production of badger interferon - gamma (IFN-γ), was also similar across the vaccinated groups.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Eamonn Gormley, Leigh A L Corner, Tara Fitzsimons, Mark A Chambers, Sandrine Lesellier, Frank E Aldwell, Eamon Costello, Denise Murphy

Experts in our system

1
E Gormley
University College Dublin
Total Publications: 68
 
2
L A L Corner
University College Dublin
Total Publications: 34
 
3
Tara Fitzsimons
University College Dublin
Total Publications: 7
 
4
Mark Chambers
University College Dublin
Total Publications: 9
 
5
Sandrine Lesellier
University College Dublin
Total Publications: 14
 
6
Frank E Aldwell
University College Dublin
Total Publications: 4
 
7
Eamon Costello
Dublin City University
Total Publications: 94
 
8
Denise Murphy
University College Dublin
Total Publications: 13