Type

Journal Article

Authors

Michael D. Gilchrist
Michel Destrade
Badar Rashid

Subjects

Physics

Topics
post mortem cold temperature tissue preservation body temperature room temperature mechanical properties elastic modulus brain tissue

Influence of preservation temperature on the measured mechanical properties of brain tissue. (2012)

Abstract The large variability in experimentally measured mechanical properties of brain tissue is due to many factors including heterogeneity, anisotropy, age dependence and post-mortem time. Moreover, differences in test protocols also influence these measured properties. This paper shows that the temperature at which porcine brain tissue is stored or preserved prior to testing has a significant effect on the mechanical properties of brain tissue, even when tests are conducted at the same temperatures. Three groups of brain tissue were stored separately for at least 1h at three different preservation temperatures, i.e., ice cold, room temperature (22 °C) and body temperature (37 °C), prior to them all being tested at room temperature (~22 °C). Significant differences in the corresponding initial elastic shear modulus μ (Pa) (at various amounts of shear, 0≤K≤1.0) were observed. The initial elastic moduli were 1043±271 Pa, 714±210 Pa and 497±156 Pa (mean±SD) at preservation temperatures of ice cold, 22 °C and 37 °C, respectively. Based on this investigation, it is strongly recommended that brain tissue samples must be preserved at an ice-cold temperature prior to testing in order to minimize the difference between the measured in vitro test results and the in vivo properties. A by-product of the study is that simple shear tests allow for large, almost perfectly homogeneous deformation of brain matter.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Michael D. Gilchrist, Michel Destrade, Badar Rashid

Experts in our system

1
M. D. Gilchrist
University College Dublin
Total Publications: 172
 
2
Michel Destrade
National University of Ireland Galway
Total Publications: 106
 
3
Badar Rashid
University College Dublin
Total Publications: 16