Type

Journal Article

Authors

Trudee Fair
Patrick Lonergan
Lorraine Brennan
Solomon Mamo
Alan G Fahey
Katrin Bender
Satoko Matoba

Subjects

Veterinary

Topics
follicular fluid fatty acids granulosa cells principal component analysis components palmitic acids bovine cumulus cells

Predictive value of bovine follicular components as markers of oocyte developmental potential. (2012)

Abstract The follicle is a unique micro-environment within which the oocyte can develop and mature to a fertilisable gamete. The aim of this study was to investigate the ability of a panel of follicular parameters, including intrafollicular steroid and metabolomic profiles and theca, granulosa and cumulus cell candidate gene mRNA abundance, to predict the potential of bovine oocytes to develop to the blastocyst stage in vitro. Individual follicles were dissected from abattoir ovaries, carefully ruptured under a stereomicroscope and the oocyte was recovered and individually processed through in vitro maturation, fertilisation and culture. The mean (±s.e.m.) follicular concentrations of testosterone (62.8±4.8ngmL(-1)), progesterone (616.8±31.9ngmL(-1)) and oestradiol (14.4±2.4ngmL(-1)) were not different (P>0.05) between oocytes that formed (competent) or failed to form (incompetent) blastocysts. Principal-component analysis of the quantified aqueous metabolites in follicular fluid showed differences between oocytes that formed blastocysts and oocytes that degenerated; l-alanine, glycine and l-glutamate were positively correlated and urea was negatively correlated with blastocyst formation. Follicular fluid associated with competent oocytes was significantly lower in palmitic acid (P=0.023) and total fatty acids (P=0.031) and significantly higher in linolenic acid (P=0.036) than follicular fluid from incompetent oocytes. Significantly higher (P<0.05) transcript abundance of LHCGR in granulosa cells, ESR1 and VCAN in thecal cells and TNFAIP6 in cumulus cells was associated with competent compared with incompetent oocytes.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Trudee Fair, Patrick Lonergan, Lorraine Brennan, Solomon Mamo, Alan G Fahey, Katrin Bender, Satoko Matoba

Experts in our system

1
T Fair
University College Dublin
Total Publications: 66
 
2
P. Lonergan
University College Dublin
Total Publications: 190
 
3
Lorraine Brennan
University College Dublin
Total Publications: 143
 
4
Solomon Mamo
University College Dublin
Total Publications: 16
 
5
A. G. Fahey
University College Dublin
Total Publications: 26