Type

Journal Article

Authors

Kevin E O'Connor
Zhi Li
Lydie Coulombel
Patrick J Guiry
Timothy O'Sullivan
Evelyn M Doyle
Leona Martin
Jasmina Nikodinovic
Sarah J Brooks

Subjects

Chemistry

Topics
chiral reducing agent alcohol mushroom production 1 protein ethanol ascorbic acid

Production of a chiral alcohol, 1-(3,4-dihydroxyphenyl) ethanol, by mushroom tyrosinase. (2012)

Abstract 1-(3,4-Dihydroxyphenyl) ethanol was produced biocatalytically for the first time using mushroom tyrosinase. 4-Ethylphenol at 1 mM was consumed over 12 min giving 0.23 mM 4-ethylcatechol and 0.36 mM (R/S)-1-(3,4-dihydroxyphenyl) ethanol (ee 0.5 %). Mushroom tyrosinase consumed 4-ethylphenol at 6.7 μmol min(-1) mg protein(-1) while the rates of formation of 4-ethylcatechol and 1-(3,4-dihydroxyphenyl) ethanol were 1.1 and 1.9 μmol min(-1) mg protein(-1). Addition of the ascorbic acid, as a reducing agent to biotransformation reactions, increased 4-ethylcatechol formation by 340 %. However, accumulation of 1-(3,4-dihydroxyphenyl) ethanol was not observed in the presence of ascorbic acid. While the 1-(3,4-dihydroxyphenyl) ethanol was racemic, it is the first chiral product produced by tyrosinase starting from a non-chiral substrate.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Kevin E O'Connor, Zhi Li, Lydie Coulombel, Patrick J Guiry, Timothy O'Sullivan, Evelyn M Doyle, Leona Martin, Jasmina Nikodinovic, Sarah J Brooks

Experts in our system

1
Kevin E O'Connor
University College Dublin
Total Publications: 52
 
2
Patrick J Guiry
University College Dublin
Total Publications: 47
 
3
Timothy P. O'Sullivan
University College Cork
Total Publications: 21
 
4
E. M. Doyle
University College Dublin
Total Publications: 34