Journal Article


D Graham
Kevin Kenny
W Cashman
S Strain
E S G Sergeant
S J More



sensitivity and specificity simulation model disease risk programming language test performance testing diagnostic tests johne s disease

The effect of alternative testing strategies and bio-exclusion practices on Johne's disease risk in test-negative herds. (2012)

Abstract Herd classification is a key component of national Johne's disease (JD) control programs. Herds are categorized on the basis of test results, and separate sub-programs are followed for test-positive and test-negative herds. However, a test-negative herd result does not necessarily equate to JD freedom for reasons relating to disease pathogenesis and available diagnostic tests. Thus, in several countries, JD control programs define test-negative herds as having a "low risk" of infection below a specified prevalence. However, the approach is qualitative, and little quantitative work is available on herd-level estimates of probability of freedom in test-negative herds. This paper examines the effect over time of alternative testing strategies and bio-exclusion practices on JD risk in test-negative herds. A simulation model was developed in the programming language R. Key model inputs included sensitivity and specificity estimates for 3 individual animal diagnostic tests (serum ELISA, milk ELISA, and fecal culture), design prevalence, testing options, and testing costs. Key model outputs included the probability that infection will be detected if present at the design prevalence or greater (herd sensitivity; SeH), the probability that infection in the herd is either absent or at very low prevalence (i.e., less than the design prevalence; ProbF), the probability of an uninfected herd producing a false-positive result [P(False+)], and mean testing cost (HerdCost) for different testing strategies. The output ProbF can be updated periodically, incorporating data from additional herd testing and information on cattle purchases, and could form the basis for an output-based approach to herd classification. A high ProbF is very difficult to achieve, reflecting the low sensitivity of the evaluated tests. Moreover, ProbF is greatly affected by any risk of introduction of infection, decreasing in herds with poor bio-exclusion practices despite ongoing negative test results. The value of P(False+) was substantial when tests with imperfect specificity were used. Testing strategies can substantially influence testing costs but with little effect on test performance. This study illustrates an output-based approach to herd classification, with potential for national and field applications.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

D Graham, Kevin Kenny, W Cashman, S Strain, E S G Sergeant, S J More

Experts in our system

David A. Graham
University College Dublin
Total Publications: 32
K Kenny
University College Dublin
Total Publications: 7
S Strain
University College Dublin
Total Publications: 6
S J More
University College Dublin
Total Publications: 213