Type

Journal Article

Authors

A W Baird
D J Brayden
P J O'Brien
D C Winter
E G Walsh
S Maher
D Collins
H M Mohan

Subjects

Pharmacology

Topics
ion transport chloride secretion epithelial cells caco 2 monolayers cell culture high content analysis epithelial permeability caco 2 cells

The mycotoxin patulin increases colonic epithelial permeability in vitro. (2012)

Abstract The gastrointestinal lumen is directly exposed to dietary contaminants, including patulin, a mycotoxin produced by moulds. Patulin is known to increase permeability across intestinal Caco-2 monolayers. This study aimed to determine the effect of patulin on permeability, ion transport and morphology in isolated rat colonic mucosae. Mucosal sheets were mounted in Ussing chambers and voltage clamped. Apical addition of patulin (100-500 μM) rapidly reduced transepithelial electrical resistance (TEER) and increased permeability to [(14)C] mannitol (2.9-fold). Patulin also inhibited carbachol-induced electrogenic chloride secretion and histological evidence of mucosal damage was observed. To examine potential mechanisms of action of patulin on colonic epithelial cells, high-content analysis of Caco-2 cells was performed and this novel, quantitative fluorescence-based approach confirmed its cytotoxic effects. With regard to time course, the cytotoxicity determined by high content analysis took longer than the almost immediate reduction of electrical resistance in isolated mucosal sheets. These data indicate patulin is not only cytotoxic to enterocytes but also has the capacity to directly alter permeability and ion transport in intact intestinal mucosae. These data corroborate and extend findings in intestinal cell culture monolayers, and further suggest that safety limits on consumption of patulin may be warranted.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

A W Baird, D J Brayden, P J O'Brien, D C Winter, E G Walsh, S Maher, D Collins, H M Mohan

Experts in our system

1
Alan W. Baird
University College Dublin
Total Publications: 70
 
2
David J Brayden
University College Dublin
Total Publications: 97
 
3
Peter J. O'Brien
University College Dublin
Total Publications: 17
 
4
Desmond C. Winter
University College Dublin
Total Publications: 53
 
5
Edwin G. Walsh
University College Dublin
Total Publications: 7
 
6
Sam Maher
University College Dublin
Total Publications: 25
 
7
Denis M Collins
Dublin City University
Total Publications: 26
 
8
Helen M Mohan
University College Dublin