Type

Journal Article

Authors

David A. Magee
Eamonn Gormley
Karsten Hokamp
Stephen DE Park
John A Browne
Nicolas C. Nalpas
Kate E Killick
Maria Taraktsoglou
David E. MacHugh

Subjects

Microbiology

Topics
systems analysis gene expression systems biology differentially expressed genes monocyte derived macrophages host pathogen interactions genomic analysis mycobacterium avium subspecies paratuberculosis

Pan-genomic analysis of bovine monocyte-derived macrophage gene expression in response to in vitro infection with Mycobacterium avium subspecies paratuberculosis. (2011)

Abstract ABSTRACT: Mycobacterium avium subspecies paratuberculosis is the causative agent of Johne's disease, an intestinal disease of ruminants with major economic consequences. Infectious bacilli are phagocytosed by host macrophages upon exposure where they persist, resulting in lengthy subclinical phases of infection that can lead to immunopathology and disease dissemination. Consequently, analysis of the macrophage transcriptome in response to M. avium subsp. paratuberculosis infection can provide valuable insights into the molecular mechanisms that underlie Johne's disease. Here, we investigate pan-genomic gene expression in bovine monocyte-derived macrophages (MDM) purified from seven age-matched females, in response to in vitro infection with M. avium subsp. paratuberculosis (multiplicity of infection 2:1) at intervals of 2 hours, 6 hours and 24 hours post-infection (hpi). Differentially expressed genes were identified by comparing the transcriptomes of the infected MDM to the non-infected control MDM at each time point (adjusted P-value threshold ≤ 0.10). 1050 differentially expressed unique genes were identified 2 hpi, with 974 and 78 differentially expressed unique genes detected 6 and 24 hpi, respectively. Furthermore, in the infected MDM the number of upregulated genes exceeded the number of downregulated genes at each time point, with the fold-change in expression for the upregulated genes markedly higher than that for the downregulated genes. Inspection and systems biology analysis of the differentially expressed genes revealed an enrichment of genes involved in the inflammatory response, cell signalling pathways and apoptosis. The transcriptional changes associated with cellular signalling and the inflammatory response may reflect different immuno-modulatory mechanisms that underlie host-pathogen interactions during infection.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

David A. Magee, Eamonn Gormley, Karsten Hokamp, Stephen DE Park, John A Browne, Nicolas C. Nalpas, Kate E Killick, Maria Taraktsoglou, David E. MacHugh

Experts in our system

1
David A. Magee
University College Dublin
Total Publications: 47
 
2
E Gormley
University College Dublin
Total Publications: 68
 
3
Karsten Hokamp
Trinity College Dublin
Total Publications: 47
 
4
John A Browne
University College Dublin
Total Publications: 73
 
5
Nicolas C. Nalpas
University College Dublin
Total Publications: 14
 
6
Kate E Killick
University College Dublin
Total Publications: 14
 
7
David E. MacHugh
University College Dublin
Total Publications: 82