Type

Journal Article

Authors

David E. MacHugh
Eamonn Gormley
Stephen V. Gordon
Karsten Hokamp
David J Lynn
Kevin M. Conlon
Stephen D. E. Park
John A Browne
Nicolas C. Nalpas
Kate E Killick
and 2 others

Subjects

Microbiology

Topics
granuloma rna monocytes macrophages systems biology oligonucleotide array sequence analysis cytology gene expression regulation animals female mycobacterium bovis cattle microbiology tuberculosis bovine gene expression profiling metabolism signal transduction transcriptome genome

Global gene expression and systems biology analysis of bovine monocyte-derived macrophages in response to in vitro challenge with Mycobacterium bovis. (2011)

Abstract Mycobacterium bovis, the causative agent of bovine tuberculosis, is a major cause of mortality in global cattle populations. Macrophages are among the first cell types to encounter M. bovis following exposure and the response elicited by these cells is pivotal in determining the outcome of infection. Here, a functional genomics approach was undertaken to investigate global gene expression profiles in bovine monocyte-derived macrophages (MDM) purified from seven age-matched non-related females, in response to in vitro challenge with M. bovis (multiplicity of infection 2:1). Total cellular RNA was extracted from non-challenged control and M. bovis-challenged MDM for all animals at intervals of 2 hours, 6 hours and 24 hours post-challenge and prepared for global gene expression analysis using the Affymetrix® GeneChip® Bovine Genome Array. Comparison of M. bovis-challenged MDM gene expression profiles with those from the non-challenged MDM controls at each time point identified 3,064 differentially expressed genes 2 hours post-challenge, with 4,451 and 5,267 differentially expressed genes detected at the 6 hour and 24 hour time points, respectively (adjusted P-value threshold ≤ 0.05). Notably, the number of downregulated genes exceeded the number of upregulated genes in the M. bovis-challenged MDM across all time points; however, the fold-change in expression for the upregulated genes was markedly higher than that for the downregulated genes. Systems analysis revealed enrichment for genes involved in: (1) the inflammatory response; (2) cell signalling pathways, including Toll-like receptors and intracellular pathogen recognition receptors; and (3) apoptosis. The increased number of downregulated genes is consistent with previous studies showing that M. bovis infection is associated with the repression of host gene expression. The results also support roles for MyD88-independent signalling and intracellular PRRs in mediating the host response to M. bovis.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

David E. MacHugh, Eamonn Gormley, Stephen V. Gordon, Karsten Hokamp, David J Lynn, Kevin M. Conlon, Stephen D. E. Park, John A Browne, Nicolas C. Nalpas, Kate E Killick and 2 others

Experts in our system

1
David E. MacHugh
University College Dublin
Total Publications: 82
 
2
E Gormley
University College Dublin
Total Publications: 68
 
3
Stephen V. Gordon
University College Dublin
Total Publications: 40
 
4
Karsten Hokamp
Trinity College Dublin
Total Publications: 47
 
5
David J Lynn
Teagasc
Total Publications: 37
 
6
Stephen D. E. Park
University College Dublin
Total Publications: 35
 
7
John A Browne
University College Dublin
Total Publications: 73
 
8
Nicolas C. Nalpas
University College Dublin
Total Publications: 14
 
9
Kate E Killick
University College Dublin
Total Publications: 14