Journal Article


Lorraine Brennan
Mary Wingfield
Fionnuala M McAuliffe
Michael J Gibney
Evelyn Cottell
Martina Wallace


Medicine & Nursing

magnetic resonance spectroscopy biological markers embryo implantation embryo culture techniques infertility therapy adult humans metabolomics metabolism oocyte retrieval treatment outcome follicular fluid least squares analysis chemistry pregnancy predictive value of tests female principal component analysis fertilization in vitro cell survival embryo transfer oocytes pathology ireland discriminant analysis

An investigation into the relationship between the metabolic profile of follicular fluid, oocyte developmental potential, and implantation outcome. (2011)

Abstract To determine whether metabolomic analysis of follicular fluid could prove a useful noninvasive technique for the selection of viable oocytes and embryos. Metabolomic analysis based on proton nuclear magnetic resonance ((1)H NMR) performed on follicular fluid collected from in vitro fertilization (IVF) patients. A university research center and a private fertility clinic. Fifty-eight women undergoing IVF treatment. Follicular fluid collected at the time of oocyte retrieval. Metabolomic profile, assessment of oocyte developmental potential and embryo viability. The metabolomic profile of follicular fluid from follicles where the oocyte resulted in a fertilized egg that failed to cleave (n = 9) was distinctly different from that where oocytes developed into early cleavage-stage embryos. Discriminating metabolites included glucose, lactate, choline/phosphocholine, and lipoproteins. Comparison of follicular fluid from women who subsequently had a positive β human chorionic gonadotropin (n = 10) to those who were unsuccessful in achieving a pregnancy (n = 12) revealed metabolic differences that were correlated to cycle outcome. Differences in the metabolite composition of follicular fluid correlate with the developmental competence of the human oocyte. Therefore, metabolomic profiling of follicular fluid may prove to be an important technique in gamete/embryo selection.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Lorraine Brennan, Mary Wingfield, Fionnuala M McAuliffe, Michael J Gibney, Evelyn Cottell, Martina Wallace

Experts in our system

Lorraine Brennan
University College Dublin
Total Publications: 166
Mary B Wingfield
University College Dublin
Total Publications: 13
Fionnuala M McAuliffe
University College Dublin
Total Publications: 176
Michael J Gibney
University College Dublin
Total Publications: 102