Type

Journal Article

Authors

Virginie W Gautier
William W Hall
Giuliano Elia
Kieran Wynne
Mohamed Ali Jarboui

Subjects

Microbiology

Topics
humans proteomics jurkat cells nuclear proteins t lymphocytes chemistry immunology cell nucleolus

Proteomic profiling of the human T-cell nucleolus. (2011)

Abstract The nucleolus, site of ribosome biogenesis, is a dynamic subnuclear organelle involved in diverse cellular functions. The size, number and organisation of nucleoli are cell-specific and while it remains to be established, the nucleolar protein composition would be expected to reflect lineage-specific transcriptional regulation of rDNA genes and have cell-type functional components. Here, we describe the first characterisation of the human T-cell nucleolar proteome. Using the Jurkat T-cell line and a reproducible organellar proteomic approach, we identified 872 nucleolar proteins. In addition to ribosome biogenesis and RNA processing networks, network modeling and topological analysis of nucleolar proteome revealed distinct macromolecular complexes known to orchestrate chromatin structure and to contribute to the regulation of gene expression, replication, recombination and repair, and chromosome segregation. Furthermore, among our dataset, we identified proteins known to functionally participate in T-cell biology, including RUNX1, ILF3, ILF2, STAT3, LSH, TCF-1, SATB1, CTCF, HMGB3, BCLAF1, FX4L1, ZAP70, TIAM1, RAC2, THEMIS, LCP1, RPL22, TOPK, RETN, IFI-16, MCT-1, ISG15, and 14-3-3τ, which support cell-specific composition of the Jurkat nucleolus. Subsequently, the nucleolar localisation of RUNX1, ILF3, STAT3, ZAP70 and RAC2 was further validated by Western Blot analysis and immunofluorescence microscopy. Overall, our T-cell nucleolar proteome dataset not only further expands the existing repertoire of the human nucleolar proteome but support a cell type-specific composition of the nucleolus in T cell and highlights the potential roles of the nucleoli in lymphocyte biology.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Virginie W Gautier, William W Hall, Giuliano Elia, Kieran Wynne, Mohamed Ali Jarboui

Experts in our system

1
Virginie W Gautier
University College Dublin
Total Publications: 14
 
2
William W Hall
University College Dublin
Total Publications: 73
 
3
Giuliano Elia
Teagasc
Total Publications: 21
 
4
Kieran Wynne
Royal College of Surgeons in Ireland
Total Publications: 45
 
5
Mohamed Ali Jarboui
University College Dublin
Total Publications: 5