Type

Journal Article

Authors

Patrick Lonergan
Fuller W Bazer
Thomas E Spencer
Trudee Fair
Paul McGettigan
Jai P Mehta
Solomon Mamo

Subjects

Biochemistry

Topics
transcriptome metabolism multigene family embryo implantation gene expression profiling embryo mammalian animals female sequence analysis rna cattle pregnancy

RNA sequencing reveals novel gene clusters in bovine conceptuses associated with maternal recognition of pregnancy and implantation. (2011)

Abstract Successful establishment and maintenance of pregnancy can be attained only through optimum conceptus-maternal cross talk. Despite significant progress in our understanding of the temporal changes in the transcriptome of the uterine endometrium, we have only a rudimentary knowledge of the genes and pathways governing growth and development of the bovine conceptus. In particular, very little information exists for the posthatching embryo and elongating conceptus. This period of development is arguably the most important, as approximately 40% of all embryonic loss occurs between Days 8 and 17 of pregnancy in cattle. Here, we describe the global transcriptome profile of the bovine conceptus at five key stages of its pre- and peri-implantation growth (Days 7, 10, 13, 16, and 19) using state-of-the-art RNA sequencing techniques. More than 287 million reads were generated at the five stages, and more than 22 700 unique transcripts were detected. Analysis of variance followed by self-organizing maps identified differentially regulated (P < 0.05) genes organized in nine gene clusters forming a sequential transcript dynamics across these developmental stages. Of particular interest, genes in clusters 3 (n = 236) and 6 (n = 1409) were significantly up-regulated on Days 16 and 19, suggesting a role in maternal recognition and initiation of implantation. This transcriptome analysis of the bovine conceptus will provide a blueprint of the dynamic changes in gene expression occurring during maternal recognition and implantation and will complement existing knowledge of the temporal changes in the endometrial transcriptome, thus facilitating a better understanding of conceptus-maternal cross talk during the peri-implantation period of pregnancy.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Patrick Lonergan, Fuller W Bazer, Thomas E Spencer, Trudee Fair, Paul McGettigan, Jai P Mehta, Solomon Mamo

Experts in our system

1
P. Lonergan
University College Dublin
Total Publications: 190
 
2
Fuller W Bazer
University College Dublin
Total Publications: 8
 
3
Thomas E Spencer
University College Dublin
Total Publications: 14
 
4
T Fair
University College Dublin
Total Publications: 66
 
5
Paul A. McGettigan
University College Dublin
Total Publications: 44
 
6
Jai Prakash Mehta
University College Dublin
Total Publications: 29
 
7
Solomon Mamo
University College Dublin
Total Publications: 16