Type

Journal Article

Authors

Pauline M Rudd
Rita M Hickey
Mariarosaria Marotta
David J Harvey
Weston B Struwe
Jodie L Abrahams
Jonathan A Lane
Karina Mariño

Subjects

Microbiology

Topics
milk cattle chromatography high pressure liquid anthranilamide animals chemistry oligosaccharides ortho aminobenzoates hydrophobic and hydrophilic interactions colostrum

Method for milk oligosaccharide profiling by 2-aminobenzamide labeling and hydrophilic interaction chromatography. (2011)

Abstract Although the properties of milk oligosaccharides have been of scientific interest for many years, their structural diversity presents a challenging analytical task. In the quest for a simple and robust technology to characterize the different oligosaccharides present in milk, we developed an analytical scheme based on their fluorescent labeling, pre-fractionation by weak anionic exchange chromatography and separation by hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC). HILIC relies on the hydrophilic potential of the molecule, which accounts for differences in properties such as molecular volume, lipophilic surface area, charge, composition, structure, linkage and oligosaccharide branching. The robustness of the methodology has been demonstrated using bovine colostrum oligosaccharides as a case study. Structural assignments for 37 free glycans, including 20 sialylated species, were obtained by a combination of HILIC-HPLC, exoglycosidase digestion and offline negative-ion mode mass spectrometry (MS)/MS. Parameters obtained for each glycan, including linkages, enzymatic digestion products and glucose unit values, will be added to GlycoBase, a public access database (http://glycobase.nibrt.ie/glycobase.html). This approach provides a basis for the analysis of free milk oligosaccharides in a fast and sensitive manner and could be adapted for an automated technology platform amenable to diverse environments. Indeed, our approach, in conjunction with bacterial-binding assays, can provide a better understanding of the structural elements required for biological activity of free milk oligosaccharides and could serve as a scientific basis for the selection of such bioactives from various food sources.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Pauline M Rudd, Rita M Hickey, Mariarosaria Marotta, David J Harvey, Weston B Struwe, Jodie L Abrahams, Jonathan A Lane, Karina Mariño

Experts in our system

1
Pauline M Rudd
University College Dublin
Total Publications: 45
 
2
Rita M. Hickey
Teagasc
Total Publications: 30
 
3
Mariarosaria Marotta
Teagasc
Total Publications: 4
 
4
Jonathan A Lane
University College Cork
Total Publications: 14