Type

Journal Article

Authors

Lorraine Brennan
Michael J Gibney
Kevin D. Cashman
Francesca Paradisi
Daniele Balducci
Aifric O'Sullivan

Subjects

Pharmacology

Topics
cholecalciferol dietary supplements adult deuterium metabolism humans glycogenolysis 1 2 o isopropylidene d glucofuranose physiology pharmacology drug effects analogs derivatives radioactive tracers female glucose liver magnetic resonance spectroscopy regression analysis

Effect of supplementation with vitamin D₃ on glucose production pathways in human subjects. (2010)

Abstract Research reports suggest that vitamin D affects glucose and insulin metabolism; however, the exact mechanisms are unclear. ²H NMR analysis of monoacetone glucose (MAG) after tracer administration provides a non-invasive method of profiling hepatic glucose metabolism. This study examined the effects of supplementation with vitamin D₃ on contribution of glycogenolysis to glucose production. Tracer administration and biofluid collections were performed with eight healthy females before and following a 4-wk vitamin D₃ administration period. Following an overnight fast subjects ingested deuterated water and acetaminophen. Full void urine samples were collected after 4 h. ²H NMR spectra of urinary monoacetone glucose were acquired to determine the contribution of glycogenolysis to glucose production. The mean contribution of glycogenolysis to glucose production was 60±13%. Supplementation with vitamin D₃ had no effect on hepatic glucose production. Regression analysis revealed a significant relationship between carbohydrate intake and the contribution of glycogenolysis (β=0.914, p=0.004). In conclusion, we saw no changes in the percentage contribution of glycogenolysis following supplementation with vitamin D₃. The reproducibility of our results and the non-invasive nature of the method highlight the potential for this method in assessing mechanistic modes of action in future nutritional interventions.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Lorraine Brennan, Michael J Gibney, Kevin D. Cashman, Francesca Paradisi, Daniele Balducci, Aifric O'Sullivan

Experts in our system

1
Lorraine Brennan
University College Dublin
Total Publications: 166
 
2
Michael J Gibney
University College Dublin
Total Publications: 102
 
3
Kevin D Cashman
University College Cork
Total Publications: 102
 
4
Francesca Paradisi
University College Dublin
Total Publications: 40
 
5
Aifric O'Sullivan
University College Dublin
Total Publications: 11