Type

Journal Article

Authors

William M Gallagher
Stephen R Pennington
Thomas Y K Lau
Ben C Collins

Subjects

Biochemistry

Topics
animals chemistry proteins trypsin isotope labeling metabolism isolation purification statistics as topic liver proteomics mass spectrometry cytology rats chromatography ion exchange

Differential proteomics incorporating iTRAQ labeling and multi-dimensional separations. (2010)

Abstract Considerable effort is currently being expended to integrate newly developed "omics"-based approaches (proteomics, transcriptomics, and metabonomics) into preclinical safety evaluation workflows in the hope that more sensitive prediction of toxicology can be achieved as reported by Waters and Fostel (Nat. Rev. Genet. 5(12):936-948, 2004) and Craig et al. (J. Proteome Res. 5(7):1586-1601, 2006). Proteomic approaches are well placed to contribute to this effort as (a) proteins are the metabolically active products of genes and, as such, may provide more sensitive and direct predictive information on drug-induced liabilities and (b) they have the potential to determine tissue leakage markers in peripheral fluids. Here, we describe a workflow for proteomic semi-quantitative expression profiling of liver from rats treated with a known hepatotoxicant using a multiplexed isobaric labeling strategy and multi-dimensional liquid chromatography.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

William M Gallagher, Stephen R Pennington, Thomas Y K Lau, Ben C Collins

Experts in our system

1
William M Gallagher
University College Dublin
Total Publications: 148
 
2
Stephen R Pennington
University College Dublin
 
3
Thomas Y K Lau
University College Dublin
Total Publications: 7
 
4
Ben C Collins
University College Dublin
Total Publications: 8