Journal Article


P. Lonergan
W A King
D Rath
A D Macaulay
D A F Villagómez
T F Carter
M Garcia-Herreros



female cattle y chromosome in situ hybridization fluorescence embryology pathology x chromosome animals veterinary embryo culture techniques male sex determination analysis chimera ploidies sex preselection chromosome aberrations blastocyst spermatozoa physiology fertilization in vitro flow cytometry

Incidence of chromosomal abnormalities in bovine blastocysts derived from unsorted and sex-sorted spermatozoa. (2010)

Abstract The aim of the present study was to examine the incidence of chromosomal abnormalities in bovine blastocysts produced by IVF with unsorted, X-sorted or Y-sorted spermatozoa. In Experiment 1, individual blastocysts were processed to examine the incidence of mixoploidy using fluorescent in situ hybridisation. Overall, 80% (44/55) of blastocysts were mixoploid (10/15, 14/15 and 20/25 for X-sorted, Y-sorted and unsorted spermatozoa, respectively; P > 0.05). However, the prevalence of abnormal XY chromosome complements was relatively low in all groups; on average, only a small fraction of the total nuclei per embryo appeared polyploid (1.64%, 5.62% and 6.0% for X-sorted, Y-sorted and unsorted spermatozoa, respectively). Interestingly, 20% (5/25) of blastocysts derived from unsorted spermatozoa were found to be chimeric (XX/XY). In Experiment 2, chimeric embryos were detected among the blastocysts derived from two of five sires tested. In addition, one chimeric blastocyst was detected among nine in vivo-derived blastocysts obtained following AI. In conclusion, based on the results of the present study, the incidence of chromosomal abnormalities did not different between blastocysts derived from sex-sorted or unsorted spermatozoa. In addition, the occurrence of mixed sex chimeras was not limited to a single sire and was not unique to blastocysts derived from IVF.
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Full list of authors on original publication

P. Lonergan, W A King, D Rath, A D Macaulay, D A F Villagómez, T F Carter, M Garcia-Herreros

Experts in our system

P. Lonergan
University College Dublin
Total Publications: 190